Bisphenol-A analogs induce lower urinary tract dysfunction in male mice.

Bisphenol-A (BPA), an estrogenic endocrine disrupting chemical, significantly impacts numerous diseases and abnormalities in mammals. Estrogens are known to play an important role in the biology of the prostate; however, little is known about the role of bisphenols in the etiology of prostate pathologies, including benign prostate hyperplasia (BPH) and associated lower urinary tract dysfunction (LUTD). Bisphenol-F (BPF) and bisphenol-S (BPS) are analogs often used as substitutes for BPA; they are both reported to have in vitro and in vivo estrogenic effects similar to or more potent than BPA. The objective of this study was to assess the role of these bisphenols in the development of LUTD in adult male mice. In adult mice exposed to BPA, BPS or BPF, we examined urinary tract histopathology and physiological events associated with urinary dysfunction. Mice treated with bisphenols displayed increased bladder (p < 0.005) and prostate (p < 0.0001) mass, and there was an increased number of prostatic ducts in the prostatic urethra (p < 0.05) and decreased size of the urethra lumen (p < 0.05) compared to negative controls. After two months of bisphenol exposure, mice displayed notable differences in cystometric tracings compared to controls, consistent with LUTD. Treatment of male mice with all bisphenols also induced voiding dysfunction manifested by detrusor instability and histologic changes in the prostatic urethra of male rodents, consistent with LUTD. Our results implicate BPA and its replacements in the development and progression LUTD in mice and provide insights into the development and progression of BPH/LUTS in men.

Effect of an intensive lifestyle intervention on the prevalence of metabolic syndrome and its components among overweight and obese adults.

BACKGROUND: Despite the fact that up to a third of the global population has metabolic syndrome (MetS), it has been overlooked in clinical settings. This study assesses the impact of a physician-supervised nonsurgical weight management program on the prevalence of MetS and its key indicators. METHODS: Four-hundred seventy-nine overweight and obese participants aged 19 years or older were included in a prospective longitudinal study. Changes in MetS and its key indicators were assessed using the binomial exact, chi-square and Wilcoxon signed-rank tests in an intent-to-treat study population. Differences in age strata were assessed using a generalized linear model. RESULTS: Fifty-two percent of participants (n = 249) had MetS at baseline. Prevalence of MetS decreased steadily with significant changes from baseline observed at weeks 13 (31.8%, P < 0.0001), 26 (28.7%, P < 0.0012) and 39 (21.6%, P < 0.0002); changes from baseline were observed at week 52 as statistically significant (16.7%, P < 0.0012). Improvements in anthropometrics and levels of key indicators of MetS were observed throughout the study. CONCLUSION: These findings confirm that weight loss is inversely associated with prevalence of MetS and its key indicators among overweight and obese individuals. Future studies may benefit from a larger sample size and better retention (ClinicalTrials.gov ID: NCT03588117).

The relationship between indoor and outdoor temperature, apparent temperature, relative humidity, and absolute humidity.

Many studies report an association between outdoor ambient weather and health. Outdoor conditions may be a poor indicator of personal exposure because people spend most of their time indoors. Few studies have examined how indoor conditions relate to outdoor ambient weather. The average indoor temperature, apparent temperature, relative humidity (RH), and absolute humidity (AH) measured in 16 homes in Greater Boston, Massachusetts, from May 2011 to April 2012 was compared to measurements taken at Boston Logan airport. The relationship between indoor and outdoor temperatures is nonlinear. At warmer outdoor temperatures, there is a strong correlation between indoor and outdoor temperature (Pearson correlation coefficient, r = 0.91, slope, ß = 0.41), but at cooler temperatures, the association is weak (r = 0.40, ß = 0.04). Results were similar for outdoor apparent temperature. The relationships were linear for RH and AH. The correlation for RH was modest (r = 0.55, ß = 0.39). Absolute humidity exhibited the strongest indoor-to-outdoor correlation (r = 0.96, ß = 0.69). Indoor and outdoor temperatures correlate well only at warmer outdoor temperatures. Outdoor RH is a poor indicator of indoor RH, while indoor AH has a strong correlation with outdoor AH year-round.

Early changes of guanylate cyclase and cGMP phosphodiesterase activities in glomeruli and tubules isolated from the remaining kidney after unilateral nephrectomy in the rabbit.

Guanylate cyclase (GCase) and cyclic guanosine 3'5'-monophosphate-phosphodiesterase (cGMP-PDE) activities were measured in the medulla, glomeruli and cortical tubules isolated from the remaining kidney 5-120 min after unilateral nephrectomy. The results showed that cGMP-PDE-specific activities were unchanged in all the fractions isolated from the contralateral kidney when compared with the nephrectomized control kidney. The GCase-specific activity in the cortical and medullary tubules isolated from the remaining kidney reached a maximum by 10 min after unilateral nephrectomy and then returned to control values. On the other hand the glomerular GCase activity decreased for 10 min after unilateral nephrectomy, then rebound activity reached a maximum by 30 min and after 120 min had returned to the control value. The possible physiological signification of such variations is discussed.